Perfecting DNA plasmid isolation: Mira-prep

The best way to isolate DNA has been discovered by: Pronobis MI, Deuitch N, Peifer M. The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields. PLoS One. 2016 Aug 3;11(8)

I adapted the protocol slightly from its original contents to include the detailed recipes for the various buffers used. The procedure with work with any type of DNA plasmid spin filter on the market and can be adapted to any type of plasmid miniprep kit.