eGFP expression in iPSC induced cardiomyocytes
"The Little Cog kit did not cause cell death, and cardiomyocytes continued beating during and immediately after transfection. With the Mirus kit, we usually see cell loss, and beating resumes only 12–24 hours after a 24-hour transfection.
With Mirus, peak expression typically occurs ~72 hours post-transfection, whereas with Little Cog, expression was observed much earlier."
- Heta Lad, MASc, Institute of Biomaterials and Biomedical Engineering, University of Toronto